Overview

  • Product name
    Rosiglitazone
  • Description
    Selective PPARγ agonist
  • Biological description
    Selective PPARγ agonist (EC50 = 43 nM). Thiazolidinedione. Mediates adipocyte differentiation, increases lipogenesis and enhances fatty acid and glucose uptake.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab3580 staining glucocorticoid receptor in serum starved HeLa cells treated with rosiglitazone (120762), by ICC/IF. Changes in localization of glucocorticoid receptor (translocation from cytoplasm to nucleous) correlates with increased concentration of rosiglitazone, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120762 (rosiglitazone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab3580 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Wang N  et al. Role of peroxisome proliferator-activated receptor-? in atherosclerosis: an update. Circ J 75:528-35 (2011). Read more (PubMed: 21325726) »
  • Chinetti G  et al. PPAR-alpha and PPAR-gamma activators induce cholesterol removal from human macrophage foam cells through stimulation of the ABCA1 pathway. Nat Med 7:53-8 (2001). Read more (PubMed: 11135616) »
  • Gouni-Berthold I  et al. Troglitazone and rosiglitazone induce apoptosis of vascular smooth muscle cells through an extracellular signal-regulated kinase-independent pathway. Naunyn Schmiedebergs Arch Pharmacol 363:215-21 (2001). Read more (PubMed: 11218074) »
  • Forman BM  et al. The peroxisome proliferator-activated receptors: ligands and activators. Ann N Y Acad Sci 804:266-75 (1996). Read more (PubMed: 8993549) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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