Overview

  • Product nameAnti-Rotavirus antibody
    See all Rotavirus primary antibodies
  • Description
    Goat polyclonal to Rotavirus
  • SpecificityThis antibody is specific against ICPs & late structural (virion) antigens. Uninfected cell reactivity is negative against HEp-2 cells and WI-38 cells by indirect immunofluorescence.
  • Tested applicationsSuitable for: ICC/IF, Conjugationmore details
  • Species reactivity
    Reacts (>90%) with Human rotaviruses.
  • Immunogen

    Bovine Nebraska Calf Diarrhea Virus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notes>95% pure.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Conjugation Use at an assay dependent dilution.

Target

  • RelevanceRotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
  • Alternative names
    • Major inner capsid protein VP6 antibody
    • NSP4 antibody
    • VP6 antibody

References for Anti-Rotavirus antibody (ab20036)

This product has been referenced in:
  • Yang Y  et al. Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. Sci China Life Sci 54:82-9 (2011). WB . Read more (PubMed: 21104033) »

See 1 Publication for this product

Product Wall

I have been in contact with the lab regarding the issues which you have been experiencing with ab20036. Per the lab, this product does stain the cytoplasma of infected cells, but it is not a strong antibody in IFA. The lab recommends using it at a 1:1...

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This is a polyclonal antibody that was raised using the whole virus, so it reacts with a number of the viral proteins. In ICC/IF, it gives cytoplasmic staining.

Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this antibody.

I can confirm the immunogen used to producethis antibodyis Rotavirus NCDV (Nebraska Calf Diarrhea Virus). I'msor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"