Overview

  • Product nameAnti-RP105 antibody
    See all RP105 primary antibodies
  • Description
    Rabbit polyclonal to RP105
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, IHC (PFA fixed), WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic 14 amino acid peptide from near the carboxy terminus of human RP105 .

  • Positive control
    • Human spleen tissue, human spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24945347
IHC-P Use a concentration of 10 µg/ml.
IHC (PFA fixed) Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 74 kDa.

Target

  • FunctionMay cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.
  • Tissue specificityExpressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 19 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD180 antibody
    • CD180 antigen antibody
    • CD180_HUMAN antibody
    • F630107B15 antibody
    • LY64 antibody
    • Ly78 antibody
    • Lymphocyte antigen 64 antibody
    • lymphocyte antigen 64 radioprotective 105kDa antibody
    • lymphocyte antigen-64, radioprotective, 105kDa antibody
    • MGC126233 antibody
    • MGC126234 antibody
    • Radioprotective 105 kDa protein antibody
    • RP105 antibody
    see all

Anti-RP105 antibody images

  • Immunohistochemistry of RP105 in human spleen tissue with ab45429 at 10 µg/ml.
  • Lane 1 : Anti-RP105 antibody (ab45429) at 0.5 µg/ml
    Lane 2 : Anti-RP105 antibody (ab45429) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 74 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-RP105 antibody (ab45429)

This product has been referenced in:
  • Bastiaansen AJ  et al. TLR4 Accessory Molecule RP105 (CD180) Regulates Monocyte-Driven Arteriogenesis in a Murine Hind Limb Ischemia Model. PLoS One 9:e99882 (2014). IHC-P, IHC-Fr ; Mouse . Read more (PubMed: 24945347) »

See 1 Publication for this product

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