Primary antibody notesCross linking of CD180 with clone RP/14 has been reported to induce resistance against irradiation induced apoptosis, B-cell proliferation and up-regulation of a co-stimulatory molecule B7.2.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl to label 106 cells in 100µl.
ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionMay cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells.
Tissue specificityExpressed mainly on mature peripherical B cells. Detected in spleen, lymph node and appendix. Not detected in pre-B and -T cells.
Sequence similaritiesBelongs to the Toll-like receptor family. Contains 19 LRR (leucine-rich) repeats. Contains 1 LRRCT domain.