Recombinant
RabMAb

Anti-RP2 antibody [EPR11362] (ab174840)

Overview

  • Product name
    Anti-RP2 antibody [EPR11362]
    See all RP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11362] to RP2
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IF,IHC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RP2 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O75695

  • Positive control
    • HeLa and Sh-SY5Y cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11362
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IF,IHC or IP.
  • Target

    • Relevance
      Function: Acts as a GTPase-activating protein (GAP) involved in trafficking between the Golgi and the ciliary membrane. Involved in localization of proteins, such as NPHP3, to the cilium membrane by inducing hydrolysis of GTP ARL3, leading to the release of UNC119 (or UNC119B). Acts as a GTPase-activating protein (GAP) for tubulin in concert with tubulin-specific chaperone C, but does not enhance tubulin heterodimerization. Acts as guanine nucleotide dissociation inhibitor towards ADP-ribosylation factor-like proteins. Tissue specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level). Disease: Retinitis pigmentosa 2 Similarity: Belongs to the TBCC family. Contains 1 C-CAP/cofactor C-like domain. PTM: Myristoylated on Gly-2; which may be required for membrane targeting. Palmitoylated on Cys-3; which may be required for plasma membrane targeting (Probable). Mutation of Cys-3 targets the protein to internal membranes.
    • Cellular localization
      Cell membrane; Lipid-anchor; Cytoplasmic side
    • Database links
    • Alternative names
      • DELXp11.3 antibody
      • KIAA0215 antibody
      • NM23 H10 antibody
      • NME10 antibody
      • Protein XRP2 antibody
      • Retinitis pigmentosa 2 (X linked recessive) antibody
      • Retinitis pigmentosa 2 antibody
      • RP 2 antibody
      • RP2 antibody
      • RP2 ARL3 GTPase activating protein antibody
      • TBCCD 2 antibody
      • TBCCD2 antibody
      • XRP2 antibody
      • XRP2 protein antibody
      • XRP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RP2 antibody [EPR11362] (ab174840) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Sh-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • Flow cytometric analysis of permeabilized HeLa cells labeling RP2 with ab174840 at a 1/100 dilution (red), or a rabbit IgG (negative) (green).

    References

    ab174840 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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