Overview

  • Product nameAnti-RPA14 antibody
    See all RPA14 primary antibodies
  • Description
    Mouse monoclonal to RPA14
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    N-terminally 6x-His tagged fusion protein encoding full-length human RPA14 expressed in E. coli.

  • Positive control
    • HeLa, Raji, NIH 3T3 cells and recombinant protein.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab6432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24267891
WB Use a concentration of 0.1 - 2 µg/ml. Predicted molecular weight: 14 kDa.
IP Use a concentration of 0.1 - 2 µg/ml.

Target

  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • p14 antibody
    • REPA 3 antibody
    • REPA3 antibody
    • Replication factor A protein 3 antibody
    • Replication protein A 14 kDa subunit antibody
    • Replication protein A3 14kD antibody
    • Replication protein A3 14kDa antibody
    • Replication protein A3 antibody
    • RF A antibody
    • RF A protein 3 antibody
    • RF-A protein 3 antibody
    • RFA protein 3 antibody
    • RFA3_HUMAN antibody
    • RP A antibody
    • RP A p14 antibody
    • RP-A p14 antibody
    • RPA 14 antibody
    • RPA 3 antibody
    • RPA p14 antibody
    • rpa3 antibody
    see all

References for Anti-RPA14 antibody (ab6432)

This product has been referenced in:
  • Toledo LI  et al. ATR prohibits replication catastrophe by preventing global exhaustion of RPA. Cell 155:1088-103 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24267891) »
  • Jády BE  et al. Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres. Mol Biol Cell 17:944-54 (2006). Human . Read more (PubMed: 16319170) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"