Overview

  • Product name
  • Description
    Rabbit polyclonal to RPA14
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 91 of RPA14 (NP_002938).

  • Positive control
    • WB: H1299, HeLaS3, MOLT4 and HepG2 cell lysates IHC-P: Human lung CA patient tumor

Properties

Applications

Our Abpromise guarantee covers the use of ab97436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function
    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • p14 antibody
    • REPA 3 antibody
    • REPA3 antibody
    • Replication factor A protein 3 antibody
    • Replication protein A 14 kDa subunit antibody
    • Replication protein A3 14kD antibody
    • Replication protein A3 14kDa antibody
    • Replication protein A3 antibody
    • RF A antibody
    • RF A protein 3 antibody
    • RF-A protein 3 antibody
    • RFA protein 3 antibody
    • RFA3_HUMAN antibody
    • RP A antibody
    • RP A p14 antibody
    • RP-A p14 antibody
    • RPA 14 antibody
    • RPA 3 antibody
    • RPA p14 antibody
    • rpa3 antibody
    see all

Images

  • All lanes : Anti-RPA14 antibody (ab97436) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 14 kDa
  • Immunohistochemical analysis of RPA14 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.

References

ab97436 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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