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Rabbit polyclonal to RPA14
Predicted to work with:
Recombinant protein fragment corresponding to a region within amino acids 1 and 91 of RPA14 (NP_002938).
WB: H1299, HeLaS3, MOLT4 and HepG2 cell lysates
IHC-P: Human lung CA patient tumor
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
1/100 - 1/250.
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Information by UniProt
REPA 3 antibody
Western blot - Anti-RPA14 antibody (ab97436)
All lanes : Anti-RPA14 antibody (ab97436) at 1/1000 dilution Lane 1 : HeLa whole cell lysate Lane 2 : HepG2 whole cell lysate Lane 3 : MOLT4 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA14 antibody (ab97436)
Immunohistochemical analysis of RPA14 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.
has not yet been referenced specifically in any publications.
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