RPA14 overexpression 293T lysate (whole cell) (ab94054)

Overview

  • Product nameRPA14 overexpression 293T lysate (whole cell)
  • Description
    RPA14 overexpression lysate
  • General notesab94054 is a 293T cell transfected lysate in which Human RPA14 has been transiently over-expressed using a pCMV-RPA14 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Applications

Our Abpromise guarantee covers the use of ab94054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

RPA14 overexpression 293T lysate (whole cell) images

  • ab94054 at 15 µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RPA14 antibody (ab58317) at 1/500 dilution

    Lane 1 : RPA14 overexpression 293T lysate (whole cell) (ab94054)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for RPA14 overexpression 293T lysate (whole cell) (ab94054)

ab94054 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94054.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"