Overview

  • Product nameAnti-RPA32/RPA2 antibody [9A1]
    See all RPA32/RPA2 primary antibodies
  • Description
    Mouse monoclonal [9A1] to RPA32/RPA2
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length Human RPA32/RPA2 produced in HEK293T cells (NP_002937).

  • Positive control
    • RPA32/RPA2-transfected HEK293T cell lysate; HepG2, HeLa, HT29, A549, COS7, Jurkat, and MCF7 cell extracts; Human breast adenocarcinoma, colon adenocarcinoma, colon, liver, lung, ovary adenocarcinoma, pancreas, pancreas carcinoma, thyroid, endometrium, endometrium adenocarcinoma, prostate, prostate carcinoma, bladder, and bladder carcinoma tissues; COS7 cells transiently transfected by RPA32/RPA2; HeLa and Jurkat cells.
  • General notesDilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

Properties

Applications

Our Abpromise guarantee covers the use of ab125681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 29 kDa.
IP Use a concentration of 4 µg/ml.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 antibody [9A1] images

  • All lanes : Anti-RPA32/RPA2 antibody [9A1] (ab125681) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RPA32/RPA2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 29 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205715)
  • All lanes : Anti-RPA32/RPA2 antibody [9A1] (ab125681) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 29 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205715)
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human bladder tissue by Immunohistochemistry.
  • ab125681 at 1/50 dilution staining RPA32/RPA2 in Paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab125681 at 1/100 dilution staining RPA32/RPA2 in COS7 cells transiently transfected with pCMV6-ENTRY RPA32/RPA2 by Immunofluorescence.
  • ab125681 at 2µg/500µl used for immunoprecipiatation of DDK tagged RPA32/RPA2 overexpression lysates (lane 2) (at 1/5 dilution with HEK293T lysate). Lane 1: Negative control (without ab125681). Secondary: 20µl (0.1mg) of goat anti-Mouse conjugated magnetic beads were mixed and incubated overnight.
  • ab125681 at 1/100 dilution staining RPA32/RPA2 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab125681 at 1/100 dilution staining RPA32/RPA2 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-RPA32/RPA2 antibody [9A1] (ab125681)

ab125681 has not yet been referenced specifically in any publications.

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