• Product nameAnti-RPA32/RPA2 antibody
    See all RPA32/RPA2 primary antibodies
  • Description
    Rabbit polyclonal to RPA32/RPA2
  • SpecificityDetects endogenous levels of total RPA32/RFA2 protein
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non phosphopeptide (Human) from around the phosphorylation site of threonine 21 (GYTPQS)

  • Positive control
    • Human lung carcinoma tissue HeLa cells



Our Abpromise guarantee covers the use of ab61184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.


  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 antibody images

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab61184 at 1/50 dilution. Samples were treated -/+ blocking peptide.
  • Immunofluorescence analysis of HeLa cells labelled with ab61184 at 1/500 dilution. Samples were treated -/+ blocking peptide.

References for Anti-RPA32/RPA2 antibody (ab61184)

This product has been referenced in:
  • Jiang Q  et al. MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links. Genes Dev 29:1955-68 (2015). Human . Read more (PubMed: 26338419) »
  • Goldstein M  et al. Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair. Proc Natl Acad Sci U S A 110:16874-9 (2013). ChIP . Read more (PubMed: 24082117) »

See all 3 Publications for this product

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