Overview

  • Product nameAnti-RPA32/RPA2 antibody
    See all RPA32/RPA2 primary antibodies
  • Description
    Mouse polyclonal to RPA32/RPA2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RPA32/RPA2 (AAH12157).

  • Positive control
    • Human kidney, placenta and stomach tissue lysates, RPA32/RPA2 transfected 293T cell lysate, human liver tissue and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 antibody images

  • Anti-RPA32/RPA2 antibody (ab88675) at 1 µg/ml + human kidney tissue lysate at 50 µg

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Anti-RPA32/RPA2 antibody (ab88675) at 1 µg/ml + human stomach tissue lysate at 50 µg

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • Anti-RPA32/RPA2 antibody (ab88675) at 1 µg/ml + human placenta tissue lysate at 50 µg

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ab88675, at 3 µg/ml staining RPA32/RPA2 in formalin-fixed, paraffin-embedded human liver by Immunohistochemistry.
  • ab88675 at 10µg/ml staining RPA32/RPA2 in HeLa cells by Immunofluorescence.
  • All lanes : Anti-RPA32/RPA2 antibody (ab88675) at 1 µg/ml

    Lane 1 : RPA32/RPA2 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

References for Anti-RPA32/RPA2 antibody (ab88675)

ab88675 has not yet been referenced specifically in any publications.

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