• Product nameAnti-RPA32/RPA2 antibody [RPA34-20]
    See all RPA32/RPA2 primary antibodies
  • Description
    Mouse monoclonal [RPA34-20] to RPA32/RPA2
  • Tested applicationsSuitable for: ICC, IHC-Fr, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Other
  • Immunogen

    Replication protein A purified from U293 cells.

  • Positive control
    • Within the p34 subunit of replication protein A. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.



Our Abpromise guarantee covers the use of ab16850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.


  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 antibody [RPA34-20] images

  • RPA32/RPA2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to RPA32/RPA2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16850.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 29kDa; RPA32/RPA2

  • IHC image of RPA32/RPA2 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16850, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Detection of human replication protein A by IF staining.
    Sample: HeLa cells fixed with methanol
    Primary antibody: Anti-Replication Protein A, Human (Mouse)
  • Staining of SKN cells with ab16850 (red) at a dilution of 1/1000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab16850. The DNA is stained with DAPI (blue). 100x magnification.

    Staining of SKN cells with ab16850 (red) at a dilution of 1/1000. The cells were fixed in paraformaldehyde for 10 minutes prior to incubation with ab16850. The DNA is stained with DAPI (blue). 100x magnification.

References for Anti-RPA32/RPA2 antibody [RPA34-20] (ab16850)

This product has been referenced in:
  • Toledo LI  et al. ATR prohibits replication catastrophe by preventing global exhaustion of RPA. Cell 155:1088-103 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24267891) »
  • Shimamura S & Ishikawa F Interaction between DNMT1 and DNA replication reactions in the SV40 in vitro replication system. Cancer Sci 99:1960-6 (2008). ChIP . Read more (PubMed: 19016755) »

See all 2 Publications for this product

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