Anti-RPA32/RPA2 antibody [SPM316], prediluted (ab17852)


  • Product nameAnti-RPA32/RPA2 antibody [SPM316], prediluted
    See all RPA32/RPA2 primary antibodies
  • Description
    Mouse monoclonal [SPM316] to RPA32/RPA2, prediluted
  • SpecificityMolecular weight: 32-34 kDa We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human).

  • EpitopeNot determined
  • Positive control
    • Hela cells. Tonsil.



Our Abpromise guarantee covers the use of ab17852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: 1/1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
  • Target

    • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
    • Post-translational
      Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
    • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • 60S acidic ribosomal protein P1 antibody
      • AA409079 antibody
      • AI325195 antibody
      • AU020965 antibody
      • ik:tdsubc_2g1 antibody
      • M(2)21C antibody
      • MGC137236 antibody
      • OTTHUMP00000004008 antibody
      • p32 antibody
      • p34 antibody
      • RCJMB04_6d17 replication protein A2, 32kDa antibody
      • REPA2 antibody
      • Replication factor A protein 2 antibody
      • Replication protein A 32 kDa subunit antibody
      • Replication protein A 32kDa subunit antibody
      • Replication protein A 34 kDa subunit antibody
      • Replication protein A antibody
      • Replication Protein A2 (32kDa) antibody
      • Replication protein A2 antibody
      • Replication protein A2, 32kDa antibody
      • RF-A protein 2 antibody
      • Rf-A2 antibody
      • RFA antibody
      • RFA2_HUMAN antibody
      • RP-A p32 antibody
      • RP-A p34 antibody
      • RP21C antibody
      • RPA 2 antibody
      • RPA 32 antibody
      • RPA antibody
      • Rpa2 antibody
      • RPA32 antibody
      • RPA34 antibody
      • RpLP1 antibody
      • RpP2 antibody
      • xx:tdsubc_2g1 antibody
      • zgc:109822 antibody
      see all

    Anti-RPA32/RPA2 antibody [SPM316], prediluted images

    • Human tonsil stained with ab17852.

    References for Anti-RPA32/RPA2 antibody [SPM316], prediluted (ab17852)

    ab17852 has not yet been referenced specifically in any publications.

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