Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)

Overview

  • Product nameAnti-RPA32/RPA2 (phospho S4 + S8) antibody
    See all RPA32/RPA2 primary antibodies
  • Description
    Rabbit polyclonal to RPA32/RPA2 (phospho S4 + S8)
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    A dually phosphorylated synthetic peptide, which represented a portion of Human RPA32/RPA2, surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1, GeneID 6118).

  • Positive control
    • Human Controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma
      Mouse Controls: Colon Carcinoma CT26
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab87277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
ICC/IF 1/100 - 1/500.

Target

  • FunctionRequired for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Post-translational
    modifications
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • Cellular localizationNucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all

Anti-RPA32/RPA2 (phospho S4 + S8) antibody images

  • Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/250 dilution, with mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right). Detection: DAB staining.
  • Immunohistochemistry analysis of Mouse colon carcinoma using ab87277 at 1/250 dilution, with mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right). Detection: DAB staining.
  • Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/100 dilution. Detection: Red-fluorescent goat anti-rabbit IgG at 1/100 dilution.

References for Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)

This product has been referenced in:
  • Liu S  et al. Distinct roles for DNA-PK, ATM and ATR in RPA phosphorylation and checkpoint activation in response to replication stress. Nucleic Acids Res : (2012). WB ; Human . Read more (PubMed: 22977173) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Breast Cancer Cell Line)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Treatment 3mM Hydroxyurea 5hrs
Specification Breast Cancer Cell Line
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (ROSA)
Loading amount 30 µg
Specification ROSA
Treatment Ad-Cre virus
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 13 2012

DISCOUNT CODE: *** Expiration date: March 29, 2012 I am very pleased to hear you would like to test ab87277 in Western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
Loading amount 35 µg
Specification Mouse Embryonic Fibroblast
Treatment 10 Gy IR
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Mr. Balaji Parameswaran

Verified customer

Submitted Dec 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"