Anti-RPA32/RPA2 (phospho S4 + S8) antibody (ab87277)
Key features and details
- Rabbit polyclonal to RPA32/RPA2 (phospho S4 + S8)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPA32/RPA2 (phospho S4 + S8) antibody
See all RPA32/RPA2 primary antibodies -
Description
Rabbit polyclonal to RPA32/RPA2 (phospho S4 + S8) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to Human RPA32/RPA2 (N terminal) (phospho S4 + S8).
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Positive control
- IHC-P: Human ovarian cancer tissue, mouse renal cancer tissue, human breast carcinoma.
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General notes
Concentration is optimized for IHC and not determined
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
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Notes |
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IHC-P
1/100 - 1/500. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. |
Target
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Function
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
Post-translational
modificationsPhosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
Cellular localization
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100172265 Orangutan
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
- SwissProt: Q62193 Mouse
see all -
Alternative names
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling RPA32 with ab87277 at a 1/250 dilution. Detection: DAB.
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Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/100 dilution. Detection: Red-fluorescent goat anti-rabbit IgG at 1/100 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab87277 has been referenced in 18 publications.
- Carley AC et al. Replication Protein A Phosphorylation Facilitates RAD52-Dependent Homologous Recombination in BRCA-Deficient Cells. Mol Cell Biol 42:e0052421 (2022). PubMed: 34928169
- Anand R et al. HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Nature 601:268-273 (2022). PubMed: 34937945
- Feng YL et al. DNA nicks induce mutational signatures associated with BRCA1 deficiency. Nat Commun 13:4285 (2022). PubMed: 35879372
- Zhang T et al. Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin. Nat Commun 13:5133 (2022). PubMed: 36050397
- Kilgas S et al. p97/VCP inhibition causes excessive MRE11-dependent DNA end resection promoting cell killing after ionizing radiation. Cell Rep 35:109153 (2021). PubMed: 34038735