Overview

  • Product nameAnti-RPA34 antibody [9H8 / 34A / alpha SSB34A]
  • Description
    Mouse monoclonal [9H8 / 34A / alpha SSB34A] to RPA34
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human RPA34 protein.

  • Positive control
    • HeLa cells.
  • General notes


    ab81191 inhibits the stimulation of pol alpha, but has no effect on DNA unwinding and pol delta stimulation (Kenny et. al., 1990).

Properties

Applications

Our Abpromise guarantee covers the use of ab81191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. For 2 hours at room temperature.
IP Use at 2 µg/mg of lysate. Native verified.
EMSA Use at an assay dependent concentration.

Target

  • RelevanceReplication Protein A (RPA) is a single stranded DNA binding protein. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
  • Cellular localizationNuclear. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Database links
  • Alternative names
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • replication protein A2 (32kD) antibody
    • replication protein A2, 32kDa antibody
    • RF A protein 2 antibody
    • RP A p32 antibody
    • RPA2 antibody
    • RPA32 antibody
    see all

Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] images

  • anti RPA34 (version of ab81191 containing BSA and Azide) at 1 µg/ml + human cells

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References for Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] (ab81191)

This product has been referenced in:
  • Kenny MK  et al. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem 265:7693-700 (1990). WB . Read more (PubMed: 2159011) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"