The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. For 2 hours at room temperature.
Use at 2 µg/mg of lysate. Native verified.
Use at an assay dependent concentration.
RelevanceReplication Protein A (RPA) is a single stranded DNA binding protein. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta.
RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV
Cellular localizationNuclear. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.