Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).
Our Abpromise guarantee covers the use of ab12320 in the following tested applications.
|IP||Use at 3-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
Detection of Human RPA70 by Western Blot.