Overview

  • Product nameAnti-RPA70 antibody
    See all RPA70 primary antibodies
  • Description
    Rabbit polyclonal to RPA70
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 350 - 400 of Human RPA70 (NP_002936.1)

  • Positive control
    • Human: breast carcinoma, colon carcinoma, stomach adenocarcinoma, testicular seminoma. Mouse: colon carcinoma CT26.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. Antigen retrieval with tris-EDTA buffer pH 9.0 recommended. Likely to work with frozen sections.
ICC/IF Use at an assay dependent concentration. PubMed: 21079677

Target

  • FunctionPlays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Sequence similaritiesBelongs to the replication factor A protein 1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dmrpa1 antibody
    • Drosophila Replication Protein A antibody
    • DRPA antibody
    • HSSB antibody
    • Human single stranded DNA binding protein antibody
    • MST075 antibody
    • MSTP075 antibody
    • p70 antibody
    • REPA1 antibody
    • Replication factor A antibody
    • Replication factor A protein 1 antibody
    • Replication protein A 70 kDa DNA-binding subunit antibody
    • Replication protein A 70kDa DNA binding subunit antibody
    • Replication protein A1 70kDa antibody
    • Replication protein A1 antibody
    • RF A antibody
    • RF-A protein 1 antibody
    • RFA antibody
    • RFA1_HUMAN antibody
    • RP A antibody
    • RP-A p70 antibody
    • RPA 70 antibody
    • RPA antibody
    • rpa1 antibody
    • Single stranded binding protein 70 antibody
    • Single-stranded DNA-binding protein antibody
    see all

Anti-RPA70 antibody images

  • ab87272 at 1/250 dilution staining RPA70 in formalin-fixed, paraffin-embedded Human testicular seminoma tissue.
    Detection: DAB staining.
  • ab87272 at 1/250 dilution staining RPA70 in formalin-fixed, paraffin-embedded Mouse colon carcinoma tissue.
    Detection: DAB staining.

References for Anti-RPA70 antibody (ab87272)

This product has been referenced in:
  • Luo M  et al. MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Nat Commun 4:2788 (2013). Read more (PubMed: 24240703) »
  • Shin YH  et al. Hormad1 mutation disrupts synaptonemal complex formation, recombination, and chromosome segregation in mammalian meiosis. PLoS Genet 6:e1001190 (2010). ICC/IF ; Mouse . Read more (PubMed: 21079677) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"