Overview

  • Product nameAnti-RPA70 antibody
    See all RPA70 primary antibodies
  • Description
    Rabbit polyclonal to RPA70
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 369 and 616 of Human RPA70 (NP_002936).

  • Positive control
    • 293T, A431, HepG2, Molt-4, and Raji cell lysates. H1299 and HeLaS3 whole cell lysate. HeLa cells for IF. Cal27 xenograft for IHC.

Properties

Applications

Our Abpromise guarantee covers the use of ab97338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • FunctionPlays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Sequence similaritiesBelongs to the replication factor A protein 1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dmrpa1 antibody
    • Drosophila Replication Protein A antibody
    • DRPA antibody
    • HSSB antibody
    • Human single stranded DNA binding protein antibody
    • MST075 antibody
    • MSTP075 antibody
    • p70 antibody
    • REPA1 antibody
    • Replication factor A antibody
    • Replication factor A protein 1 antibody
    • Replication protein A 70 kDa DNA-binding subunit antibody
    • Replication protein A 70kDa DNA binding subunit antibody
    • Replication protein A1 70kDa antibody
    • Replication protein A1 antibody
    • RF A antibody
    • RF-A protein 1 antibody
    • RFA antibody
    • RFA1_HUMAN antibody
    • RP A antibody
    • RP-A p70 antibody
    • RPA 70 antibody
    • RPA antibody
    • rpa1 antibody
    • Single stranded binding protein 70 antibody
    • Single-stranded DNA-binding protein antibody
    see all

Anti-RPA70 antibody images

  • All lanes : Anti-RPA70 antibody (ab97338) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 68 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA70 antibody (ab97338) at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA70 antibody (ab97338) at 1/100 dilution.

References for Anti-RPA70 antibody (ab97338)

This product has been referenced in:
  • Marin-Vicente C  et al. RRP6/EXOSC10 is required for the repair of DNA double-strand breaks by homologous recombination. J Cell Sci 128:1097-107 (2015). Read more (PubMed: 25632158) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"