Overview

  • Product name
  • Description
    Rabbit polyclonal to RPA70
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 215-264 of Human RPA70 (NP_002936).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • Sequence similarities
    Belongs to the replication factor A protein 1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Dmrpa1 antibody
    • Drosophila Replication Protein A antibody
    • DRPA antibody
    • HSSB antibody
    • Human single stranded DNA binding protein antibody
    • MST075 antibody
    • MSTP075 antibody
    • p70 antibody
    • REPA1 antibody
    • Replication factor A antibody
    • Replication factor A protein 1 antibody
    • Replication protein A 70 kDa DNA-binding subunit antibody
    • Replication protein A 70kDa DNA binding subunit antibody
    • Replication protein A1 70kDa antibody
    • Replication protein A1 antibody
    • RF A antibody
    • RF-A protein 1 antibody
    • RFA antibody
    • RFA1_HUMAN antibody
    • RP A antibody
    • RP-A p70 antibody
    • RPA 70 antibody
    • RPA antibody
    • rpa1 antibody
    • Single stranded binding protein 70 antibody
    • Single-stranded DNA-binding protein antibody
    see all

Images

  • Anti-RPA70 antibody (ab98266) at 1/1 dilution + HeLa cell lysate at 10 µg/ml

    Predicted band size : 68 kDa

References

ab98266 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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