Overview

  • Product name
    Anti-RPAIN antibody
  • Description
    Rabbit polyclonal to RPAIN
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 17 amino acid synthetic peptide conjugated to KLH corresponding to the C terminus of Human RPAIN.

  • Positive control
    • Human Adrenal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab118508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.
  • Tissue specificity
    Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis, prostate, thymus and spleen.
  • Sequence similarities
    Contains 1 RIP-type zinc finger.
  • Post-translational
    modifications
    Isoform 2 is sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues.
  • Cellular localization
    Cytoplasm. Nucleus and Nucleus > PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2400006N03Rik antibody
    • DN-393H17.1 antibody
    • FLJ25625 antibody
    • FLJ30490 antibody
    • FLJ42429 antibody
    • HRIP antibody
    • MGC4189 antibody
    • Nuclear transporter antibody
    • RAP interaction protein antibody
    • RIP antibody
    • RIP_HUMAN antibody
    • RP23 83I13.5 antibody
    • RPA interacting protein antibody
    • RPA-interacting protein antibody
    • Rpain antibody
    see all

Images

  • ab118508 at 5 µg/ml staining RPAIN in Formalin-Fixed, Paraffin-Embedded Human adrenal tissue, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab118508 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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