• Product name
  • Description
    Rabbit polyclonal to RPB2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 4 (LocusLink ID 5431).

  • Positive control
    • Whole cell lysate from 293T cells.



Our Abpromise guarantee covers the use of ab10332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
IP Use a concentration of 2 - 5 µg/ml.


  • Relevance
    This 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
  • Cellular localization
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II 140 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit B antibody
    • hRPB140 antibody
    • hsRPB2 antibody
    • POL2RB antibody
    • Polymerase (RNA) II (DNA directed) polypeptide B (140kD) antibody
    • RNA polymerase II 140kD subunit antibody
    • RNA polymerase II second largest subunit antibody
    • RNA polymerase II subunit 2 antibody
    • RNA polymerase II subunit B2 antibody
    see all

Anti-RPB2 antibody images

  • Predicted band size : 134 kDa

    Detection of RPB2 using ab10332 by Western blot. Samples: Whole cell lysate (50 mg) from 293 cells. From left to right: ab10332 concentrations 1, 0.5, 0.5 and 2mg/ml; two left hand columns after 1 minute’s exposure and two right hand columns after 2 hours’ exposure. Band representing RPB2 at approximately 140kDa.
  • Detection of RPB2 using ab10332 by immunoprecipitation. Samples: Whole cell lysate from 293 cells. Antibodies: ab10332 used at 10mg/10cm IP plate. Detection: chemiluminescence with exposure time of 10 seconds.

References for Anti-RPB2 antibody (ab10332)

This product has been referenced in:
  • Brem R  et al. Reactive oxygen species generated by thiopurine/UVA cause irreparable transcription-blocking DNA lesions. Nucleic Acids Res 37:1951-61 (2009). WB ; Human . Read more (PubMed: 19208641) »

See 1 Publication for this product

Product Wall

The immunogen for this antibody is a peptide between residues 75 and 125 of human RNA Polymerase 2 Polypeptide B using the numbering given in entry NP_000929.1. That is, it is a peptide contained within the following range:


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