Overview

  • Product nameAnti-RPB2 antibody
    See all RPB2 primary antibodies
  • Description
    Rabbit polyclonal to RPB2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 6 and 142 of Human RPB2 (NP_000929).

  • Positive control
    • A431, H1299, HeLa or HepG2 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab96797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 134 kDa.
ICC/IF 1/100 - 1/200.

Target

  • RelevanceThis 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II 140 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit B antibody
    • hRPB140 antibody
    • hsRPB2 antibody
    • POL2RB antibody
    • Polymerase (RNA) II (DNA directed) polypeptide B (140kD) antibody
    • RNA polymerase II 140kD subunit antibody
    • RNA polymerase II second largest subunit antibody
    • RNA polymerase II subunit 2 antibody
    • RNA polymerase II subunit B2 antibody
    see all

Anti-RPB2 antibody images

  • Anti-RPB2 antibody (ab96797) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 134 kDa
  • Top: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96797 at 1/200 dilution.
    Bottom: image merged with DNA probe.
  • Immunohistochemistry analysis of paraffin-embedded NCIN87 xenograft using ab96797 at 1/500 dilution.

References for Anti-RPB2 antibody (ab96797)

ab96797 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"