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Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 11 (LocusLink ID 5431).
Our Abpromise guarantee covers the use of ab10338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration. PubMed: 21252857|