• Product name
    Anti-RPB2 antibody - ChIP Grade
    See all RPB2 primary antibodies
  • Description
    Rabbit polyclonal to RPB2 - ChIP Grade
  • Tested applications
    Suitable for: WB, IP, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Human, Arabidopsis thaliana
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Caenorhabditis elegans, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 11 (LocusLink ID 5431).

  • Positive control
    • Whole cell lysate from 293 cells. IF: DU145 cell line



Our Abpromise guarantee covers the use of ab10338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 21252857


  • Relevance
    This 140 kDa protein is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. It is suggested that Rpb2 plays a role in enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.
  • Cellular localization
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II 140 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit B antibody
    • hRPB140 antibody
    • hsRPB2 antibody
    • POL2RB antibody
    • Polymerase (RNA) II (DNA directed) polypeptide B (140kD) antibody
    • RNA polymerase II 140kD subunit antibody
    • RNA polymerase II second largest subunit antibody
    • RNA polymerase II subunit 2 antibody
    • RNA polymerase II subunit B2 antibody
    see all


  • Predicted band size : 140 kDa

    Detection of RBP2 by Western blot and immunoprecipitation using ab10338. Sample: Whole cell lysate (50mg for Western blot; 10cm plate for IP) from 293 cells.Antibodies: ab10338 used at the indicated concentration (A) 1mg/ml (B) or 10mg/10cm plate for IP. Detection: ECL with exposure times as indicated (A) or 10 seconds (B).
  • ICC/IF image of ab10338 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10338, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Liu Y  et al. snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. PLoS Biol 14:e1002571 (2016). ChIP ; Arabidopsis thaliana . Read more (PubMed: 27780203) »
  • Raczynska KD  et al. FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors. Nucleic Acids Res 43:9711-28 (2015). ChIP . Read more (PubMed: 26250115) »

See all 9 Publications for this product

Customer reviews and Q&As

Western blot
Oxytricha trifallax Cell lysate - whole cell (Whole Cell Protein Lysate)
Gel Running Conditions
Non-reduced Denaturing (20% SDS-PAGE)
Loading amount
500 µg
Whole Cell Protein Lysate
Blocking step
Roche Western Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C


Verified customer

Submitted Apr 29 2015

Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Arabidopsis thaliana Tissue lysate - whole (Flower subcellular fraction)
Flower subcellular fraction
Blocking step
Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 13 2014

Thank you for contacting Abcam.

The immunogen sequence for ab10338 is a region between residues *********of human RNA Polymerase 2 Polypeptide B using the numbering given in entry NP_000929.1 (GeneID 5431). We do not know the exact immunogen ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Purified protein (purified human RNA polymerase II complex)
Loading amount
0.2 µg
purified human RNA polymerase II complex
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 21 2008


Sign up