Overview

  • Product nameAnti-RPC62 antibody
    See all RPC62 primary antibodies
  • Description
    Rabbit polyclonal to RPC62
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 484-534 of Human RPC62 (NP_006459.3)

  • Positive control
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab112057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 61 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similaritiesBelongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed III 62 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit C antibody
    • DNA-directed RNA polymerase III subunit rpc3 antibody
    • POLR3C antibody
    • Polymerase (RNA) III (DNA directed) polypeptide C (62kD) antibody
    • RNA polymerase C subunit 3 antibody
    • RNA polymerase III 62 kDa subunit antibody
    • RNA polymerase III C62 subunit antibody
    • RNA polymerase III subunit C3 antibody
    • RPC3 antibody
    • RPC3_HUMAN antibody
    • RPC62 antibody
    see all

Anti-RPC62 antibody images

  • All lanes : Anti-RPC62 antibody (ab112057) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 61 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of RPC62. 1 mg of HeLa Whole cell lysate IP in which 20% of IP was loaded. Blotted with rabbit anti-RPC62 antibody (ab112057) at 0.4 µg/ml. Lane 1- RPC62 was immunoprecipitated by rabbit anti- RPC62 antibody (ab112057) at 6 mcg/mg lysate; Lane 2- RPC62 was immunoprecipitated by an rabbit anti- RPC62 antibody, that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure times of 3 minutes.

References for Anti-RPC62 antibody (ab112057)

ab112057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"