Overview

  • Product nameAnti-RPC62 antibody
    See all RPC62 primary antibodies
  • Description
    Mouse polyclonal to RPC62
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RPC62 protein (NP_006459.3).

  • Positive control
    • WB: HeLa nuclear extract, RPC62 transfected 293T cell lysate ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 61 kDa).

Target

  • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
  • Sequence similaritiesBelongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed III 62 kDa polypeptide antibody
    • DNA-directed RNA polymerase III subunit C antibody
    • DNA-directed RNA polymerase III subunit rpc3 antibody
    • POLR3C antibody
    • Polymerase (RNA) III (DNA directed) polypeptide C (62kD) antibody
    • RNA polymerase C subunit 3 antibody
    • RNA polymerase III 62 kDa subunit antibody
    • RNA polymerase III C62 subunit antibody
    • RNA polymerase III subunit C3 antibody
    • RPC3 antibody
    • RPC3_HUMAN antibody
    • RPC62 antibody
    see all

Anti-RPC62 antibody images

  • Anti-RPC62 antibody (ab88243) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RPC62 antibody (ab88243) at 1 µg/ml

    Lane 1 : RPC62 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
  • ab88243, at 10 µg/ml, staining human RPC62 in HeLa cell by Immunofluorescence.

References for Anti-RPC62 antibody (ab88243)

ab88243 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your message. Following to our previous correspondence, I would like to update you that we have re-tested this antibody in Western blot for HeLa nuclear lysate and IF for HeLa cell (on non-transfected cells) and the results are positi...

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Thank you for your response. The protocol used to test this antibody for ICC/IF is a standard immunostaining procedure and you can find some useful information at our Protocol page: http://www.abcam.com/index.html?pageconfig=resource&rid=11...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. After reading through the detailed protocol you kindly ...

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