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Tissue, cells or virus corresponding to RPE65. Bovine RPE microsomal membrane proteins.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab13826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000. PubMed: 170320581/1000 (from PubMed:17032058).|
|WB||1/5000 - 1/10000. Detects a band of approximately 65 kDa.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21494877
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ARPE19 (spontaneously arising human retinal pigment epithelia cell line) cells stained for RPE65 (green) using ab13826 (1/100 dilution) in ICC/IF.
Cells were fixed in 4% PFA for 10 minutes, permeabilized in PBS containing 0.2% Triton X-100 (PBS-T) for 10 minutes, then blocked for 1 hour in 10% normal goat serum containing 1% BSA in PBS-T. Incubated with primary antibody at 4°C.
Frozen sectioned mouse retina tissue stained for RPE65 with ab13826 at a 1/250 dilution in immunohistochemical analysis.
Flow Cytometry analysis of mouse retina and choroid cells stained for RPE65 using ab13826.
RPE65 (right) versus control (left) in the choroid (top) and the retina (bottom).