Overview

  • Product nameAnti-RPE65 antibody
    See all RPE65 primary antibodies
  • Description
    Rabbit polyclonal to RPE65
  • SpecificityThis antibody do not cross react with other retinal proteins.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Mouse RPE65 aa 127-146.
    Sequence:

    EVTDNALVNIYPVGEDYYAC

Properties

Applications

Our Abpromise guarantee covers the use of ab74998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: >1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
    • Tissue specificityRetinal pigment epithelium specific.
    • Involvement in diseaseDefects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
      Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
    • Sequence similaritiesBelongs to the carotenoid oxygenase family.
    • Post-translational
      modifications
      Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
    • Cellular localizationCytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • All-trans-retinyl-palmitate hydrolase antibody
      • LCA 2 antibody
      • LCA2 antibody
      • Leber congenital amaurosis antibody
      • mRPE 65 antibody
      • mRPE65 antibody
      • p63 antibody
      • rd 12 antibody
      • rd12 antibody
      • Retinal pigment epithelium specific 61 kDa protein antibody
      • Retinal pigment epithelium specific 65 kDa protein antibody
      • Retinal pigment epithelium specific protein antibody
      • Retinal pigment epithelium specific protein 65kDa antibody
      • Retinal pigment epithelium-specific 65 kDa protein antibody
      • Retinitis pigmentosa 20 antibody
      • Retinoid isomerohydrolase antibody
      • Retinol isomerase antibody
      • RP 20 antibody
      • RP20 antibody
      • RPE 65 antibody
      • RPE65 antibody
      • RPE65_HUMAN antibody
      • sRPE 65 antibody
      • sRPE65 antibody
      see all

    References for Anti-RPE65 antibody (ab74998)

    ab74998 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"