Overview

  • Product name
  • Description
    Rabbit polyclonal to RPF1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: SGKKSLKRKA AAEELQEAAG AGDGATENGV QPPKAAAFPP GFSISEIKNK QRRHLMFTRW KQQQRKEKLA AKKKLKKERE ALGDKAPPKP VPKTID, corresponding to N terminal amino acids 11-106 of Human RPF1 (UniProt ID: Q9H9Y2).

  • Positive control
    • IHC-P: Human hippocampus tissue WB: RT-4 and U-251 MG cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab121833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be required for ribosome biogenesis.
  • Sequence similarities
    Contains 1 Brix domain.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310066N05Rik antibody
    • BBR140 antibody
    • Brix domain containing 5 antibody
    • Brix domain-containing protein 5 antibody
    • BXDC5 antibody
    • DKFZp761G0415 antibody
    • DKFZp761M0215 antibody
    • FLJ12475 antibody
    • Ribosome biogenesis protein RPF1 antibody
    • Ribosome production factor 1 antibody
    • Ribosome production factor 1 homolog (S. cerevisiae) antibody
    • RNA processing factor 1 (RPF1) antibody
    • RP11-118B23.1 antibody
    • RPF1 antibody
    • RPF1_HUMAN antibody
    see all

Images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-RPF1 antibody (ab121833) at 1/250 dilution

    Lane 1 : RT-4 cell line lysate
    Lane 2 : U-251 MG cell line lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate
  • ab121833, at a 1/20 dilution, staining RPF1 in paraffin embedded Human hippocampus tissue by Immunohistochemistry.

References

ab121833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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