Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-162 of Human RPL11 (Uniprot P62913)

  • Positive control
    • HeLa and Mouse liver whole cell lysates, Paraffin-embedded HeLa xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab155793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML.
  • Involvement in disease
    Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein L5P family.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L11 antibody
    • Cell growth inhibiting protein 34 antibody
    • Cell growth inhibiting protein34 antibody
    • CLL associated antigen KW 12 antibody
    • CLL associated antigen KW12 antibody
    • CLL-associated antigen KW-12 antibody
    • DBA 7 antibody
    • DBA7 antibody
    • GIG 34 antibody
    • GIG34 antibody
    • L11 antibody
    • Ribosomal protein L11 antibody
    • RL11_HUMAN antibody
    • RPL 11 antibody
    • Rpl11 antibody
    see all

Images

  • Anti-RPL11 antibody (ab155793) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 20 kDa



    12% SDS PAGE
  • Anti-RPL11 antibody (ab155793) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 20 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded HeLa xenograft labeling RPL11 with ab155793 at 1/500 dilution.

References

ab155793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Total embryos)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
50 µg
Specification
Total embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 23 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
600000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (S2 cells)
Specification
S2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Subhendu Roy Choudhury

Verified customer

Submitted Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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