Overview

  • Product name
    Anti-RPL18A antibody [6G6G10]
    See all RPL18A primary antibodies
  • Description
    Mouse monoclonal [6G6G10] to RPL18A
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Purified recombinant fragment, corresponding to amino acids 50-176 of Human RPL18A, expressed in E. coli (UniProt: Q02543).

  • Positive control
    • Human recombinant RPL18A protein; HEK293, HL60, Jurkat, Raji, MOLT4 and HeLa cell lysates; mouse NIH 3T3 cell lysate; HEK293 and HepG2 cells; Human kidney tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR124794-7 are from Tissue Culture Supernatant

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    6G6G10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

Images

  • Anti-RPL18A antibody [6G6G10] (ab166711) at 1/500 dilution + recombinant Human RPL18A protein

    Predicted band size : 21 kDa
  • All lanes : Anti-RPL18A antibody [6G6G10] (ab166711) at 1/500 dilution

    Lane 1 : mouse NIH 3T3 cell lysate
    Lane 2 : HEK293 cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : MOLT4 cell lysate
    Lane 7 : HeLa cell lysate


    Predicted band size : 21 kDa
  • Flow cytometric analysis of RPL18A-labeled HEK293 cells (green) and negative control cells (purple), using ab166711 at a 1/200 dilution.
  • Immunofluorescence analysis of HepG2 cells, labeling RPL18A (green) using ab166711 at a 1/200 dilution. DNA was stained with the fluoresacent dye DRAQ5 (blue).
  • Immunohistochemical analysis of paraffin-embedded, DAB-stained Human kidney tissue, labeling RPL18A using ab166711 at a 1/200 dilution.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab166711.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up