Overview

  • Product name
    Anti-RPL23A antibody - Carboxyterminal end
    See all RPL23A primary antibodies
  • Description
    Rabbit polyclonal to RPL23A - Carboxyterminal end
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human RPL23A (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • CEM, Hela, and HepG2 cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71341 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    This protein binds to a specific region on the 26S rRNA.
  • Sequence similarities
    Belongs to the ribosomal protein L23P family.
  • Post-translational
    modifications
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L23A antibody
    • FLJ27455 antibody
    • L23A antibody
    • MDA20 antibody
    • Melanoma differentiation-associated gene 20 antibody
    • Ribosomal protein L23a antibody
    • RL23A_HUMAN antibody
    • RPL23A antibody
    see all

Images

  • All lanes : Anti-RPL23A antibody - Carboxyterminal end (ab71341) at 1/60 dilution

    Lane 1 : CEM cell line lysate
    Lane 2 : Hela cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Datta D  et al. Nucleolar GTP-binding Protein-1 (NGP-1) Promotes G1 to S Phase Transition by Activating Cyclin-dependent Kinase Inhibitor p21 Cip1/Waf1. J Biol Chem 290:21536-52 (2015). Read more (PubMed: 26203195) »
  • Kim DH  et al. Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun 6:6843 (2015). Read more (PubMed: 25880450) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab71341.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up