Anti-RPL29 antibody (ab67196)
Key features and details
- Mouse polyclonal to RPL29
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL29 antibody
See all RPL29 primary antibodies -
Description
Mouse polyclonal to RPL29 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RPL29 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human pancreas tissue lysate and 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 18 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 18 kDa). |
Target
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Sequence similarities
Belongs to the ribosomal protein L29e family. - Information by UniProt
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Database links
- Entrez Gene: 6159 Human
- Omim: 601832 Human
- SwissProt: P47914 Human
- Unigene: 425125 Human
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Alternative names
- 60S ribosomal protein L29 antibody
- Cell surface heparin binding protein HIP antibody
- Cell surface heparin-binding protein HIP antibody
see all
Images
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Anti-RPL29 antibody (ab67196) at 1/500 dilution + human pancreas tissue lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RPL29 antibody (ab67196) at 1/500 dilution
Lane 1 : RPL29 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab67196 has been referenced in 1 publication.
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404