Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-171 of Human RPL3 (UniProt ID: P39023).

  • Positive control
    • A549 cells; Human AdCA xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab154882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-RPL3 antibody (ab154882) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol fixed A549 cells, labeling RPL3 using ab154882 at 1/500 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded Human AdCA xenograft tissue, labeling RPL3 using ab154882 at 1/100 dilution.

References

ab154882 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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