Overview

  • Product nameAnti-RPL30 antibody
    See all RPL30 primary antibodies
  • Description
    Mouse monoclonal to RPL30
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human RPL30 protein (AAH32700) with a 26kDa tag.

  • Positive control
    • RPL30 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 1ng/ml as a capture antibody when used against the immunogen.

Target

Anti-RPL30 antibody images

  • Anti-RPL30 antibody (ab77328) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 13 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RPL30 antibody (ab77328) at 5 µg/ml

    Lane 1 : RPL30 transfected 293T cell lysate
    Lane 2 : Non transfected 29T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 13 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged RPL30 is approximately 1ng/ml using ab77328 as a capture antibody.

References for Anti-RPL30 antibody (ab77328)

ab77328 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"