Anti-RPL34 antibody (ab122255)
Key features and details
- Rabbit polyclonal to RPL34
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL34 antibody -
Description
Rabbit polyclonal to RPL34 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRG, corresponding to N terminal amino acids 1-55 of Human RPL34
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Positive control
- Human pancreas tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.
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Notes |
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IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. |
Target
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Sequence similarities
Belongs to the ribosomal protein L34e family. - Information by UniProt
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Database links
- Entrez Gene: 6164 Human
- SwissProt: P49207 Human
- Unigene: 438227 Human
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Alternative names
- 60S ribosomal protein L34 antibody
- L34 antibody
- MGC111005 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab122255 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122255, at 1/10, staining RPL34 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
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All lanes :
Lane 1 : Marker (15, 25, 35, 55, 70, 100, 130, 250 kDa)
Lane 2 :Anti-RPL34 antibody (ab122255)
Predicted band size: 13 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122255 has not yet been referenced specifically in any publications.