Overview

  • Product nameAnti-RPL36A antibody
    See all RPL36A primary antibodies
  • Description
    Mouse monoclonal to RPL36A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Xenopus laevis, Zebrafish, Baboon, Orangutan
  • Immunogen

    Recombinant fragment: TRRTFCKKCG KHQPHKVTQY KKGKDSLYAQ GKRRYDRKQS GYGGQTKPIF RKKAKTTKKI VLRLECVEPN CRSKRMLAIK RCKHFELGGD KKRKGQVIQF, corresponding to amino acids 7-106 of Human RPL36A (NP_066357) with a tag of 26 KDa.

  • Positive control
    • PC-12, NIH/3T3, HeLa and Raw 264.7 lysates. Recombinant tagged human RPL36A fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged RPL36A is approximately 0.1ng/ml when used as a capture antibody.

Target

Anti-RPL36A antibody images

  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + Recombinant tagged human RPL36A fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 12 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + HeLa lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + PC-12 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + Raw 264.7 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • Anti-RPL36A antibody (ab77733) at 1 µg/ml + NIH/3T3 lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 12 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged RPL36A is approximately 0.1ng/ml using ab77733 as a capture antibody at 1ug/well.

References for Anti-RPL36A antibody (ab77733)

ab77733 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77733.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"