Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL5
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    A synthetic peptide corresponding to a region within amino acids 247-297 of Human RPL5 (NP_000960.2).

  • Positive control
    • 293T; HeLa; Jurkat and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 34 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
  • Involvement in disease
    Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein L18P family.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L5 antibody
    • DBA6 antibody
    • L5 antibody
    • MSTP030 antibody
    • PPP1R135 antibody
    • protein phosphatase 1, regulatory subunit 135 antibody
    • Ribosomal protein L5 antibody
    • RL5 antibody
    • RL5_HUMAN antibody
    • RP L5 antibody
    • RPL 5 antibody
    • RPL5 antibody
    • U21 RNA antibody
    • U21RNA antibody
    see all

Images

  • All lanes : Anti-RPL5 antibody (ab157099) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 34 kDa


    Exposure time : 10 seconds
  • Detection of RPL5 by Western Blot of Immunprecipitate.
    Lane 1: ab157099 at 1µg/ml labelling RPL5 in 293T whole cell lysate immunoprecipitated using ab157099 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab157099 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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