Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (LVIQDKNKYN TPKYRMIVRV TNRDIICQIA YARIEGDMIV CAAYAHELPK) of Human RPL5 (NP_000960).

  • Positive control
    • Human fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
  • Involvement in disease
    Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein L18P family.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60S ribosomal protein L5 antibody
    • DBA6 antibody
    • L5 antibody
    • MSTP030 antibody
    • PPP1R135 antibody
    • protein phosphatase 1, regulatory subunit 135 antibody
    • Ribosomal protein L5 antibody
    • RL5 antibody
    • RL5_HUMAN antibody
    • RP L5 antibody
    • RPL 5 antibody
    • RPL5 antibody
    • U21 RNA antibody
    • U21RNA antibody
    see all

Images

  • Anti-RPL5 antibody (ab90698) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
  • Anti-RPL5 antibody (ab90698) at 1/1000 dilution + Arabidopsis thaliana extract at 30 µg

    Secondary
    anti-rabbit HRP at 1/15000 dilution

    Predicted band size : 34 kDa


    Exposure time : 1 minute

References

ab90698 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Total embryonic extract)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
50 µg
Specification
Total embryonic extract
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted May 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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