Anti-RPL7A antibody (ab155147)
Key features and details
- Rabbit polyclonal to RPL7A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL7A antibody
See all RPL7A primary antibodies -
Description
Rabbit polyclonal to RPL7A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 15-296 of Human RPL7A (UniProt P62424).
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Positive control
- A431, Jurkat, Raji, Neuro2A, GL261 and C8D30 whole cell lysates; A431 cells; Human lung adenocarcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Involvement in disease
Note=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene. -
Sequence similarities
Belongs to the ribosomal protein L7Ae family. - Information by UniProt
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Database links
- Entrez Gene: 417158 Chicken
- Entrez Gene: 513128 Cow
- Entrez Gene: 6130 Human
- Entrez Gene: 27176 Mouse
- Entrez Gene: 296596 Rat
- Entrez Gene: 443912 Xenopus laevis
- Entrez Gene: 447981 Xenopus tropicalis
- Entrez Gene: 337010 Zebrafish
see all -
Alternative names
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
see all
Images
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All lanes : Anti-RPL7A antibody (ab155147) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
12% SDS PAGE -
All lanes : Anti-RPL7A antibody (ab155147) at 1/3000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RPL7A with ab155147 at 1/500 dilution.
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Immunofluorescent analysis of methanol-fixed A431 cells labeling RPL7A with ab155147 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155147 has been referenced in 2 publications.
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404
- Cagnetta R et al. Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring. Mol Cell 73:474-489.e5 (2019). PubMed: 30595434