Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL7A
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 215 and the C-terminus (residue 266) of human RPL7A (NP_000963.1).

  • Positive control
    • Whole cell lysate from HeLa, MCF7 or 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling RPL7A with ab70753 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RPL7A antibody (ab70753) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 20 µg
    Lane 2 : HeLa whole cell lysate at 4 µg
    Lane 3 : MCF7 whole cell lysate at 20 µg
    Lane 4 : 293T whole cell lysate at 20 µg

    Developed using the ECL technique

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

References

This product has been referenced in:
  • Yang PY  et al. Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities. J Am Chem Soc 132:656-66 (2010). Read more (PubMed: 20028024) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70753.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up