Overview

  • Product name
  • Description
    Goat polyclonal to RPL8
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide:

    KAGRAYHKYKAKRNC

    , corresponding to internal sequence amino acids 181-194 of Human RPL8.

  • Positive control
    • IHC-P: Human brain (cortex) and spleen tissues WB: HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 28 kDa.
ELISA 1/128000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-RPL8 antibody images

  • Anti-RPL8 antibody (ab116239) at 0.01 µg/ml + HepG2 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa


    Exposure time : 1 hour
  • ab116239, at 2.5 µg/ml, staining RPL8 in formalin fixed, paraffin embedded Human brain (cortex) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab116239, at 2.5 µg/ml, staining RPL8 in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated anti-goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References for Anti-RPL8 antibody (ab116239)

ab116239 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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