Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-244 of Human RPL8 (Uniprot ID P62917).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt4, Raji and mouse brain whole cell lysates, A431 cells, NCIN87 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.01% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RPL8 antibody (ab155136) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Molt4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Anti-RPL8 antibody (ab155136) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling RPL8 with ab155136 at 1/200. Image at the bottom costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded NCIN87 xenograft labeling RPL8 with ab155136 antibody at 1/100.

References

ab155136 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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