Overview

  • Product nameAnti-RPL8 antibody
    See all RPL8 primary antibodies
  • Description
    Rabbit polyclonal to RPL8
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP, corresponding to C terminal amino acids 181-230 of Human RPL8

  • Positive control
    • HepG2 cell lysate or human lung tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-RPL8 antibody images

  • Anti-RPL8 antibody (ab56256) at 0.3125 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling RPL8 with ab56256 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

     

References for Anti-RPL8 antibody (ab56256)

ab56256 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab56256.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"