Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL9
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

Target

Images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 21 kDa
    Observed band size : 21 kDa

References

This product has been referenced in:
  • Miettinen TP & Björklund M Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions. Nucleic Acids Res 43:1019-34 (2015). Read more (PubMed: 25550424) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
200000 cells
Specification
S2 Drosophila cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Marianthi Kiparaki

Verified customer

Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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