Customer reviews and FAQs

Overview

  • Product nameAnti-RPL9 antibody
    See all RPL9 primary antibodies
  • Description
    Rabbit polyclonal to RPL9
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

Target

Anti-RPL9 antibody images

  • Western blot - RPL9 antibody (ab50384)
    Western blot - RPL9 antibody (ab50384)
    Lane 1 : Molecular weight markers
    Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 21 kDa
    Observed band size : 21 kDa

References for Anti-RPL9 antibody (ab50384)

ab50384 has not yet been referenced specifically in any publications.

Publishing research using ab50384? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-RPL9 antibody

 Good
Abreviews
Application Western blot
Sample Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 200000 cells
Specification S2 Drosophila cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Username

Gerard Rimesso

Verified customer

Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"