Overview

  • Product nameAnti-RPL9 antibody
    See all RPL9 primary antibodies
  • Description
    Rabbit polyclonal to RPL9
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

Target

Anti-RPL9 antibody images

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 21 kDa
    Observed band size : 21 kDa

References for Anti-RPL9 antibody (ab50384)

ab50384 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Drosophila melanogaster Cell lysate - whole cell (S2 Drosophila cells)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 200000 cells
Specification S2 Drosophila cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Gerard Rimesso

Verified customer

Submitted Jul 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"