Overview

  • Product nameAnti-RPLP0 antibody
    See all RPLP0 primary antibodies
  • Description
    Mouse polyclonal to RPLP0
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length human RPLP0; amino acids 1-317 (NP_000993.1)

  • Positive control
    • Jurkat cell lysate, RPLP0 transfected 293T cell lysate and HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionRibosomal protein P0 is the functional equivalent of E.coli protein L10.
  • Sequence similaritiesBelongs to the ribosomal protein L10P family.
  • Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 36B4 antibody
    • 60S acidic ribosomal protein P0 antibody
    • 60S ribosomal protein L10E antibody
    • Acidic ribosomal phosphoprotein P0 antibody
    • Arbp antibody
    • L10E antibody
    • LP0 antibody
    • MGC107165 antibody
    • MGC107166 antibody
    • MGC111226 antibody
    • MGC88175 antibody
    • OTTMUSP00000015585 antibody
    • P0 antibody
    • PRLP0 antibody
    • Ribosomal phosphoprotein, acidic, P0 antibody
    • Ribosomal protein P0 antibody
    • Ribosomal protein, large, P0 antibody
    • RLA0_HUMAN antibody
    • rplP0 antibody
    • RPP0 antibody
    see all

Anti-RPLP0 antibody images

  • Anti-RPLP0 antibody (ab88872) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • All lanes : Anti-RPLP0 antibody (ab88872) at 1 µg/ml

    Lane 1 : RPLP0 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab88872 at 10µg/ml staining RPLP0 in HeLa cells by Immunofluorescence.

References for Anti-RPLP0 antibody (ab88872)

This product has been referenced in:
  • Deblois G  et al. ERRa mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun 7:12156 (2016). WB ; Human . Read more (PubMed: 27402251) »
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . Read more (PubMed: 24658146) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"