Anti-RPLP0 antibody [EP15646] (ab192866)
Key features and details
- Rabbit monoclonal [EP15646] to RPLP0
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-RPLP0 antibody [EP15646]
See all RPLP0 primary antibodies -
Description
Rabbit monoclonal [EP15646] to RPLP0 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, Jurkat, A375, C6, RAW264.7, PC12 and NIH 3T3 cell lysates; mouse kidney, mouse spleen, rat brain and rat spleen lysates; Human stomach tissue, mouse liver tissue, rat cerebral cortex tissue; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP15646 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/1000. |
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Ribosomal protein P0 is the functional equivalent of E.coli protein L10. -
Sequence similarities
Belongs to the ribosomal protein L10P family. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 6175 Human
- Entrez Gene: 11837 Mouse
- Entrez Gene: 64205 Rat
- Omim: 180510 Human
- SwissProt: P05388 Human
- SwissProt: P14869 Mouse
- SwissProt: P19945 Rat
- Unigene: 546285 Human
see all -
Alternative names
- 36B4 antibody
- 60S acidic ribosomal protein P0 antibody
- 60S ribosomal protein L10E antibody
see all
Images
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All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/20000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab192866 has been referenced in 4 publications.
- Harding HP et al. The ribosomal P-stalk couples amino acid starvation to GCN2 activation in mammalian cells. Elife 8:N/A (2019). PubMed: 31749445
- Lopez CK et al. Ontogenic Changes in Hematopoietic Hierarchy Determine Pediatric Specificity and Disease Phenotype in Fusion Oncogene-Driven Myeloid Leukemia. Cancer Discov 9:1736-1753 (2019). PubMed: 31662298
- Matthes F et al. Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis. Cell Death Discov 4:4 (2018). PubMed: 29531801
- Conotte S et al. Metabonomic profiling of chronic intermittent hypoxia in a mouse model. Respir Physiol Neurobiol N/A:N/A (2018). WB . PubMed: 29522877