Anti-RPLP2 antibody (ab154958)
Key features and details
- Rabbit polyclonal to RPLP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPLP2 antibody -
Description
Rabbit polyclonal to RPLP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Monkey -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-99 of Human RPLP2 (P05387).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji whole cell lysates; Human colon carcinoma tissue; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 12 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 12 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Plays an important role in the elongation step of protein synthesis. -
Sequence similarities
Belongs to the ribosomal protein L12P family. - Information by UniProt
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Database links
- Entrez Gene: 286853 Cow
- Entrez Gene: 6181 Human
- Entrez Gene: 101044500 Monkey
- Entrez Gene: 67186 Mouse
- Omim: 180530 Human
- SwissProt: P42899 Cow
- SwissProt: P05387 Human
- SwissProt: P99027 Mouse
see all -
Alternative names
- 2700049I22Rik antibody
- 60S acidic ribosomal protein P2 antibody
- Acidic ribosomal phosphoprotein P2 antibody
see all
Images
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All lanes : Anti-RPLP2 antibody (ab154958) at 1/1000 dilution
Lane 1 : Molt4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa
15% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling RPLP2 with ab154958 at 1/500.
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Immunofluorescence analysis of methanol fixed A431 cells labeling RPLP2 with ab154958 at 1/500. Lower panel shows costaining with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154958 has been referenced in 2 publications.
- Campos RK et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
- Campos RK et al. RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation. J Virol 91:N/A (2017). PubMed: 27974556