Overview

  • Product name
  • Description
    Rabbit polyclonal to RPLP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-99 of Human RPLP2 (P05387).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt4, Raji whole cell lysates; Human colon carcinoma tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RPLP2 antibody (ab154958) at 1/1000 dilution

    Lane 1 : Molt4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling RPLP2 with ab154958 at 1/500.
  • Immunofluorescence analysis of methanol fixed A431 cells labeling RPLP2 with ab154958 at 1/500. Lower panel shows costaining with Hoechst 33342.

References

This product has been referenced in:
  • Campos RK  et al. RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation. J Virol 91:N/A (2017). Read more (PubMed: 27974556) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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