Overview

  • Product name
    Anti-RPP25 antibody
  • Description
    Rabbit polyclonal to RPP25
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ASLSVLKNVP GLAILLSKDA LDPRQPGYQP PNPHPGPSSP PAAPASKRSL GEPAAGEGSA KRSQPEP, corresponding to Human RPP25.

  • Positive control
    • Human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab126338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • Function
    Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.
  • Sequence similarities
    Belongs to the histone-like Alba family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20374 antibody
    • Ribonuclease P protein subunit p25 antibody
    • RNase P protein subunit p25 antibody
    • Rpp25 antibody
    • RPP25_HUMAN antibody
    see all

Images

  • Lane 1 :
    Lane 2 : Anti-RPP25 antibody (ab126338)

    Lane 1 : Marker
    Lane 2 : SK-MEL-30 cell lysate


    Predicted band size : 21 kDa
  • ab126338, at a dilution of 1/50, staining RPP25 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab126338.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References

ab126338 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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